ISOLASI DAN UJI PENGHAMBATAN α-GLUKOSIDASE METABOLIT SEKUNDER EKSTRAK n-HEKSANA TANAMAN MIANA HIJAU-MERAH (Coleus hybridus)

Abstract

Green-red miana plant (Coleus hybridus) is an ornamental plant that is widely used as a medicinal plant. This plant belongs to genus Coleus and the family Lamiaceae. The goal of this study was to isolate secondary metabolites of green-red miana (Coleus hybridus) and analyze the inhibition activity of α-glucosidase by in vitro method. This plant was extracted using maceration method with methanol and partitioned with n-hexane and ethyl acetate. The separation of n-hexane extract was carried out using vacuum liquid chromatography followed by separation using flash chromatography. Purity of the compound was analyzed using TLC and melting point 138-140oC. The solids were recrystallized to obtain white crystalline solids with weight of 90,36 mg (coded CHHM-H-V4-FL14). Characterization of the solids using FTIR spectroscopy showed the presence of OH groups, aliphatic CH, methyl (CH3) C=C cyclic alkenes and CO. The result of the characterization with the FTIR as well as phytochemical analysis of the CHHM-H-V4-FL14 were assumed to be a steroid secondary metabolites. Analysis of the inhibition of the α-glucosidase enzyme revealed that the CHHM-H-V4- FL14 percentage of inhibition was 8.441% while the percentage of acarbose inhibition (positive control) was 99.492%. It is estimated that the green-red miana (Coleus hybridus) possesses weak inhibition activity against the α-glucosidase enzyme.