AMPLIFIKASI GEN CaM Penicillium sp. LBKURCC34 SECARA POLYMERASE CHAIN REACTION

Abstract

The fungal isolate Penicillium sp. LBKURCC34 is one of the isolates that has been successfully isolated from peat soil in the core zone of the Giam Siak Kecil-Bukit Batu Biosphere Reserve (GSKBB), Riau Province. Fungal isolates have the potential to produce secondary metabolites with antimicrobial activity, and it is important to correctly know the species of the isolate for inventory reasons, ease of secondary metabolite isolation, purification and utilization. In this study, as a first step toward the molecular identification of Penicillium sp. LBKURCC34, the fungal chromosomal DNA was amplified in the CaM region using the Polymerase Chain Reaction (PCR) method. A horizontal electrophoresis process was carried out to detect the success of the amplification process and calculate the molecular weight or length of the DNA products. A PCR product corresponding to CaM amplified region was successfully obtained after 35 cycles with the following conditions: 45 seconds of template denaturation at 94oC, 45 seconds of primer annealing at 55oC, and 1 minute of primer elongation at 72oC. The final products were subjected to a final elongation for 10 minutes at 72o C. The PCR product of the CaM amplified region of Penicillium sp. LBKURCC34 had a DNA length of 785 bps.