FRAKSINASI EKSTRAK ETIL ASETAT DAN UJI KROMATOGRAFI LAPIS TIPIS DARI FERMENTASI KO-KULTUR Penicillium sp. LBKURCC34 DAN Staphylococcus aureus

Abstract

Penicillium sp. LBKURCC34 is a fungus that can produce bioactive secondary metabolites so that it can be used as a candidate for antimicrobial compounds producer. This study aimed to initiate separation of antimicrobial compounds from secondary metabolites of co-culture fermentation Penicillium sp. LBKURCC34 and Staphylococcus aureus. The ethyl acetate extract of the co-culture fermentation was obtained from liquid-liquid extraction of fermentation media and ethyl acetate solvent. That crude extract was then fractionated using vacuum liquid chromatography (VLC). Thin layer chromatography (TLC) test was performed on crude extract and all fractions with ethyl acetate – n-hexane (7:3) and ethyl acetate – methanol (3:1) as eluents. Fractionation of crude extract from vacuum liquid chromatography produced 15 fractions with different spot patterns seen under 254 nm UV lamp. Keywords: antimicrobial, co-culture, secondary metabolites