Abstract:
Pyrazoline is a secondary metabolic compound with five circular heterocyclic structures that has
three carbon atoms and two nitrogen atoms position close to each other. Pyrazoline has high
toxicity and possess various bioactivities such as antitumor and anticancer agent. Pyrazoline (5-
(4-bromophenyl)-3-(1-hydroxynaphthalene-2-yl)-1-phenyl-4,5-dihydro-1H-pyrazole compound
has successfully synthesized with two phases of reaction. The reaction begins formation of
chalcone 3-(4-bromophenyl)-1-(1-hydroxynaphthalene-2-yl)-2-propen-1-on by reacting 1-
hydroxy-2-acetylnaphthalene compound with 4-bromobenzaldehide. Chalcone synthesis through
claisen-schmidt condensation reactions with yield 85,86%. The next step was reacted chalcone
with phenilhydrazine to produce target compound used sodium hydoxide catalyst. The target
compound synthesis method was sealed-vessel reactor used monowave then produce yield
41,07%. Pyrazoline compound ware purified by recrystallization then tested for purity with TLC
test, melting point and HPLC analysis and confirming its structure based on spectroscopic data
of UV and FTIR. The synthesized product was screened for toxicity capability by brine shrimp
lethality test (BSLT) using Artemia salina Leach larvae. Pyrazoline compounds have LC50 value
for pyrazoline are 8,38 μg/mL which can be interpreted as the synthesized compounds are very
toxic.
