SINTESIS DAN UJI TOKSISITAS SENYAWA N'1,N'3-BIS ((E,Z)-4-BROMOBENZILIDEN)MALONOHIDRAZID

Abstract

Hydrazone is a class of organic compound with the structure R1R2C=NNH2. Hydrazone have an azomethine group (-NHN=CH-) which is an aldehyde and ketone derivative, in which the oxygen atom is replaced by an =NNH2 group. The purpose of this study was to synthesize the compound N'1,N'3-bis((E,Z)-4-bromobenziliden)malonohydrazid and to determine the toxicity of the compound using the BSLT method. This compound was synthesized using the method of heating and stirring (reflux) at 50-60°C. The purity of the compound was determined using TLC test, melting point measurement and HPLC analysis. The structure of the synthesized compound was confirmed through analysis spectroscopic UV and FTIR. The result of this research is that the compound DB-4Br was successfully synthesized with a weight of 1.254 g and the yield of 93.10 %. The toxicity of compound DB-4Br was evaluated by Brine Shrimp Lethality Test (BSLT) method with shrimp larvae (Artemia salina Leach). LC50 value obtained was 59.74 g/mL. So it can be concluded that the synthesized compounds are toxic with moderate levels of toxicity because the LC50 values are >1 and <100 g/mL