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IDENTIFIKASI GEN tet BAKTERI TANAH GAMBUT HUTAN ALAMI CAGAR BIOSFER GIAM SIAK KECIL BUKIT BATU DIISOLASI DENGAN MEDIA ANTIBIOTIK

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dc.contributor.author Amalia, Aisyah Sabrina
dc.date.accessioned 2021-12-27T02:59:07Z
dc.date.available 2021-12-27T02:59:07Z
dc.date.issued 2021-06
dc.identifier.other wahyu sari yeni
dc.identifier.uri https://repository.unri.ac.id/handle/123456789/10343
dc.description.abstract Bacterial isolates named LBKURCC419, LBKURCC423 and LBKURCC424 are natural forest soil bacteria isolated using tetracycline and amoxicillin media from the Giam Siak Kecil Bukit Batu Biosphere Reserve, Riau Province. In this research, these bacteria were assessed by molecular biological techniques to know whether the isolates contain antibiotic resistance genes especially tet (tetracycline). The bacterial chromosomal DNA was isolated using the TIANamp DNA bacteria kit, then continued to determine the genes encoding tetracycline resistance using polymerase chain reaction (PCR) with eight primers of the tet genes according to the respective annealing temperature. PCR products were identified by electrophoresis. The results showed the eight tet genes were identified negative that meant the isolates did not have that gene. This can occur because the tested resistant genes may be present in plasmids while in this study perhaps the isolated DNA came from chromosomes so that it did not contain the resistant genes tested. In addition, the nature of low resistance to bacteria because it comes from natural soil also causes the mechanism of bacterial resistance to be unknown. en_US
dc.description.provenance Submitted by wahyu sari yeni (ayoe32@ymail.com) on 2021-12-27T02:59:06Z No. of bitstreams: 1 Aisyah Sabrina Amalia_compressed.pdf: 201638 bytes, checksum: ff029c2e90cdc8daa5536e0caec9061c (MD5) en
dc.description.provenance Made available in DSpace on 2021-12-27T02:59:07Z (GMT). No. of bitstreams: 1 Aisyah Sabrina Amalia_compressed.pdf: 201638 bytes, checksum: ff029c2e90cdc8daa5536e0caec9061c (MD5) Previous issue date: 2021-06 en
dc.description.sponsorship Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau Kampus Binawidya, Pekanbaru, 28293, Indonesia en_US
dc.language.iso en en_US
dc.publisher perpustakaan UR en_US
dc.subject resistance gene en_US
dc.subject soil en_US
dc.subject tetracycline AB en_US
dc.title IDENTIFIKASI GEN tet BAKTERI TANAH GAMBUT HUTAN ALAMI CAGAR BIOSFER GIAM SIAK KECIL BUKIT BATU DIISOLASI DENGAN MEDIA ANTIBIOTIK en_US
dc.type Article en_US
dc.contributor.supervisor Nurulita, Yuana


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