ANALISIS TIGA METODE PENENTUAN AKTIVITAS ENZIM α-AMILASE

Abstract

The enzyme activity as a biocatalyst can be calculated based on the number of moles of the substrate that is hydrolyzed into product per unit time (Iodine method), or the number of moles of product produced per unit time (2,3-dinitrosalicylic acid (DNS) method and the Nelson-Somogyi (NS) method). This third method are with experimental conditions of pH 7, temperature of 50°C and an incubation time of 30 minutes. The results showed that the α-amylase enzyme activity measured by the DNS method was 0.115 ± 0.002 μmol/minute and the NS method was 0.041 ± 0.025 μmol/minute, while the Iodine method had activity of 2.090 ± 0.005 μmol/minute. The activity data of these three methods after being statistically tested by the Duncan method turned out to be significant between the DNS method (2,3-dinitrosalicylic acid) with the Nelson-Somogyi (NS) method and the Iodine method.