ISOLASI DAN UJI TOKSISITAS SENYAWA METABOLIT SEKUNDER DARI EKSTRAK n-HEKSANA TUMBUHAN PAKU AHAKA (Vittaria elongata Sw)

Abstract

elongata Sw ferns is one of the fern plants known as ahaka fern area wich is widely distributed in kampar and pelalawan, Riau Province. The study was to isolate and then characterize the pure compounds obtained from extract n-hexana and to determine the toxicity of the secondary metabolites compound by the Brine Shrimp Lethality Test (BSLT) method. Extraction techniques were applied by using maceration method with methanol, continued fractionation by partition using nhexane. Secondary metabolites compounds were separated by using liquid chromatography and flash chromatography, then the pure compounds were characterized by FTIR. A compound obtained the result of isolation in the form of the brownish white amorphous solid with melting point of 219-221˚C. Of toxicity test that is good on n-hexane extract with the value of the LC₅ₒ was showed 161,163 μg/mL, from the data above was concluded that compound was toxic.