POLA TIC (Total Ion Current) LC-MS/MS EKSTRAK ETIL ASETAT METABOLIT SEKUNDER DARI MEDIA PERTUMBUHAN Penicillium sp. LBKURCC34 DENGAN VARIASI WAKTU INKUBASI

Abstract

Liquid Chromatography Mass Spectrometry (LC-MS / MS) is an analytical technique that combines the physical separation capabilities of liquid chromatography with the specificity of mass spectrometry detection. This method can identify the differences of compound from the extracts. The ethyl acetate extracts produced from the co-culture of Penicillium sp. LBKURCC34 and Staphylococcus aureus with various incubation times (8 days, 11 days and 14 days) were identified with LC-MS. The results of secondary metabolites obtained showed that the peak of the dominant compound in extracts generally was at retention times ranging from 9-13 min. The data showed that the compound characteristics are more polar. Interestingly, only extract from 14 days incubation contained less polarity compound.