ISOLASI DAN UJI TOKSISITAS SENYAWA METABOLIT SEKUNDER DARI FRAKSI 4 DAN 5 VLC EKSTRAK n-HEKSANA DAUN RUKAM (Flacourtia rukam)

Abstract

Flacourtia rukam is one of the species of Flacourtiaceae which is widely spread in Indonesia. This plant commonly used as traditional medicine by various tribes in Indonesia. The study is to isolate secondary metabolite compound from combine fraction with Vacuum Liquid Chromatography (VLC) method and Flash Chromatography method from sub fraction F3. The purity of the compound was analyzed by TLC and melting point. The melting point was obtained at 120-122oC. The isolated compound from extract n-hexane rukam leaf was characterized by FT-IR spectroscopy, which showed the presence of the OH group, CH2 (methylene), CH3 (mehyl), aliphatic C-H and C-O (alcohol). FR-H-02 revealed as a steroid β-Sitosterol after the spectroscopy data were compared with published data. Combine fraction was toxic, based on the toxicity test showed the LC50 values of 776,24 ppm. Toxicity test of β-Sitosterol showed that LC50 value >1,000 ppm which indicated the compound was not toxic against Artemia salina .