Browsing by Author "Dahliaty, Andi"

Now showing 1 - 14 of 14
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    ANALISIS INHIBISI DARI INFUSA DAUN DOLAR RAMBAT (Ficus pumila) DAN DAUN JAMBU BIJI (Psidium guajava) TERHADAP AKTIVITAS α-AMILASE
    (2016-10-12) Fresga, Roy; Dahliaty, Andi; Devi, Silvera
    α-Amylase is an enzyme that serves as a catalyst of starch hydrolysis to produce maltose in the digestive system. Inhibition of this enzyme can reduce blood glucose levels, especially in type 2 diabetic patients. Thus, the objective of this study were to determine inhibition of aqueous extract (infusa) from climbing fig (Ficus pumila) and guava leaves (Psidium guajava) in fresh and dried condition to α-amylase activities. Inhibition of the samples to α-amylase was determined by Nelson-Somogyi method. The absorbance was measured using spectrophotometry UV-Vis at 540 nm. Acarbose was used as positive control. The results showed that samples were potential inhibitors for α-amylase, i.e., infusa of climbing fig dried (91.91 + 2.43) and infusa of guava dried leaves (84.68 + 5.80). Percent inhibition of these plants were not significantly different from acarbose 0.5% (92.96 ± 0.18).
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    OPTIMASI pH DAN SUHU PADA REAKSI ENZIMATIS LAKASE Trichoderma asperellum LBKURCC1 HASIL FRAKSINASI PENGENDAPAN 20-80% KEJENUHAN AMMONIUM SULFAT
    (Elfitra, 2022-11) Nadyani, Kartina; Dahliaty, Andi
    Laccase is enzyme can be produced by the fungus Trichoderma asperellum LBKURCC1 that was isolated from cacao rhizosphere soil in Riau plantation area. This study aimed to determine the optimum pH and temperature for enzymatic reaction of T. asperellum LBKURCC1 laccase after purification of crude extract using precipitation ammonium sulfate with a concentration of 20-80%. The resulting precipitate was dialyzed using ultrafiltration (UF) centrifugation with 30.000 Dalton Molecular Weight Cut Off (MWCO) membrane to obtain partly purified enzymes. Enzyme activity was measured with a pH variation of acetate buffer pH 4,0; 4.0; 4.5; 5.0; 5.5 and phosphate buffer pH 6.0; 6.5; 7.0; 7.5; 8.0 and temperature 30-60oC using buffer obtained from measuring the optimum pH of laccase. Measuring the enzyme activity using the substrate 2,2'-azinobis-3- ethylbenzothiazolin-6-sulfonate (ABTS) at a wavelength 420 nm. The results showed that the optimum laccase activity was found at pH 5.5 (69.50±17.78) U/L and temperature 45oC (332.87±8.89) U/L.
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    PENENTUAN AKTIVITAS ENZIM LAKASE T. asperellum LBKURCC1 PADA BEBERAPA VARIASI pH dan SUHU MENGGUNAKAN MICROPLATE READER λ 405 nm
    (Elfitra, 2022-10) Cheline, Afmeritha; Dahliaty, Andi
    Laccase (EC 1.10.3.2, p-diphenol:dioxygen oxidoreductase) is a coppercontaining polyphenol oxidase. The laccase enzyme can be produced by fungi. One of the fungi is Trichoderma asperellum. T. asperellum LBKURCC1 is one of the fungi that isolated from the cocoa plantations. The activity of the laccase enzyme is influenced by several factors, including pH and temperature. The purpose of this research was to determine the activity of the laccase enzyme at various pH and temperature using a microplate reader λ 405 nm. In this research, laccase was partially purified by the precipitation method of ammonium sulfate 20-80% and dialysis using ultrafiltration centrifugation (UF) with a membrane of 30,000 Dalton Molecular Weight Cut Of (MWCO) and the activity of the laccase enzyme was tested using the Microplate reader method. Based on this research, the highest activity of the laccase enzyme T. asperellum LBKURCC1 on ABTS was found to be at optimum pH (5.5 and 4.0) and an optimum temperature of 45oC, giving a laccase activity of (65.16 ± 14.26 and 59.83 ± 5.25) and (224.66 ± 17.39) U/L respectively.
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    PENENTUAN AKTIVITAS ENZIM LAKASE T. asperellum LBKURCC1 YANG TELAH DIFRAKSINASI SECARA PARSIAL MENGGUNAKAN AMONIUM SULFAT
    (2021-03) Amalita, Lisna; Dahliaty, Andi
    Laccase are extracellular enzymes containing metal ions which used oxygen compounds to catalyze the oxidations process of various aromatic and nonaromatic compounds. The purpose of the study was to purify and determining laccase spesific activity from the isolate fungus T. asperellum LBKURCC1. Laccase enzymes purification process was carried out by teo steps, that was fractionation with ultrafiltraton membranes 10.000 MWCO (Molecular Weight Cut Of) and saturated with the addition of ammonium sulfate salts with saturation variations of 0-20%, 20- 40%, 40-60% and 60-80%. Based on this research, the results obtained were the activity of crude extract laccase of 0,01955 U/L, and highest enzymes activity was the enzyme purified by ammonium sulfat salt 0-20% saturation fraction of 0,03055 U/L. Specific activity of crude extract laccase enzyme was obtained at 4,4353 U/mg and the specific activity of purified enzymes decreased 2,1962 U/mg with a yield of 157,1296%. The results showed that enzymes in the saturation fraction of 0-20% ammonium sulfate was high activity and purity.
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    PENENTUAN TOTAL KONSENTRASI ANTOSIANIN DARI UBI JALAR UNGU (Ipomoea batatas L.) DENGAN METODE pH DIFERENSIAL SPEKTROFOTOMETRI
    (2016-05-02) Octaviani, Dinda Yulia; Nugroho, Titania Tjandrawati; Dahliaty, Andi
    Purple sweet potato (Ipomoea batatas L.) is potential as a natural antioxidant based on the high concentration of anthocyanins contained therein. The purpose of this study was to determine the total concentration of anthocyanins from purple sweet potato experiencing incubation buffer acetate using spectrophotometric differential pH method. Samples were incubated in acetate buffer at 40oC for 60 hours at a speed of 170 rpm. The process is continued by adding methanol to 70% that has been acidified with HCl 1% so that the solvent to be pH 3.5. Extracts were obtained and diluted in pH 1 and pH 4.5 solvents and then the concentration of anthocyanin determined based by the spectrophotometric differential pH method. By this method it could be determined that the total anthocyanin concentration extracted was (120.64±44.234) mg/100 g.
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    PENGARUH ION LOGAM Cu2+ DAN Mg2+ PADA AKTIVITAS LAKASE FRAKSI FLOW THROUGH KROMATOGRAFI GEL FILTRASI
    (Elfitra, 2023-11) Sinaga, Hana Hasanah; Nugroho, Titania T.; Dahliaty, Andi
    Laccase Trichoderma asperellum LBKURCC1 has been partially purified fractionation using ammonium sulfate at 0-80% saturation then gel filtration chromatography. The results of the gel filtration have different activities and purity levels The fraction has lower activity and lower purity is called laccase flow through gel filtration chromatography. The activity of the laccase enzyme was tested using substrates 2,2'-azinobis-3- ethylbenzothiazoline- 6-acid sulfonate (ABTS) measured at a wavelength of 405 nm using a microplate reader. The activity of laccase flow through is 144,02±2,71 U/L. The laccase activity was increased significantly (p≤ 0.05) to (362 ± 48)% and (445 ± 59)% compared to controls, when the enzyme was incubated for 10 minutes with 0.5 mM and 5 mM Cu2+ respectively. A significant increase (p≤ 0.05) in laccase activity was also observed when the enzyme was incubated for 10 minutes in 0.5 mM and 5 mM Mg2+, The laccase activity was increased significantly (p≤ 0.05) to (343 ± 59)% and (219 ± 50)% compared to controls.
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    PENGARUH PENAMBAHAN EDTA DAN SDS TERHADAP AKTIVITAS ENZIM LAKASE B Trichoderma asperellum LBKURCC1
    (Elfitra, 2023-12) Wati, Laras; Dahliaty, Andi; Nugroho, Titania Tj
    Laccase enzyme is an extracellular enzyme that is catalyzing lignin degradation process. This study aims to analyze the effect of the addition of EDTA and SDS compounds on laccase enzyme activity with concentration variations of 1 mM and 10 mM. Laccase enzyme from Trichoderma asperellum LBKURCC1 had been partially purified by 0-80% ammonium sulfate fractionation and gel filtration chromatography where the results of this partial purification produced two kinds of laccase, namely laccase A and laccase B. This study used laccase B enzyme. The activity of laccase B enzyme was determined by UV-Vis spectrophotomoter at 420 nm wavelength using ABTS (2,2'-Azinobis-3-ethylbenzothiazolin-6- sulfonate) as substrate. The activity of laccase B enzyme was 144.02±2.71 U/L. Addition by 1 mM EDTA was not significantly different (p>0.05) with relative activity of (100.47±6.47)% control. A significant decrease in laccase activity (p≤0.05) occurred in the addition by EDTA 10 mM, SDS 1 mM and SDS 10 mM with relative activities of (76.58±2.6)%, (38.81±3.59)%, (8.98±1.35)% of the control, respectively.
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    PENGARUH PENAMBAHAN ION LOGAM DAN PELARUT ORGANIK TERHADAP AKTIVITAS ENZIM LAKASE HASIL FRAKSINASI 20-80% AMMONIUM SULFAT
    (Elfitra, 2022-12) Widati, Retna Gian; Dahliaty, Andi
    Laccase is an enzyme that requires additional chemical components (non-protein) such as metal ions to increase its activity. This study aims to determine the effect of adding Cu2+, Mg2+ and Zn2+ metal ions and organic solvents acetonitrile, acetone, ethanol and methanol on the activity of the laccase enzyme produced from the fungus Trichoderma asperellum LBKURCC1 using ABTS as a substrate. The concentration of metal ions and organic solvents added were respectively (1, 5 and 10 mM) and (5, 20 and 40 %). Laccase enzyme activity test was measured using a UV-Vis spectrophotometer with a wavelength of 420 nm and incubated for 5 minutes. The highest laccase activity was obtained by adding Cu2+ metal ions at a concentration of 10 mM with a value of 544.00 ± 12.56 U/L, which increased laccase activity by 63,43% which acts as a cofactor. Meanwhile, the addition of organic solvents decreased its activity by 80,17% with a value of 66.00 ± 4.09 U/L in acetonitrile solvent with a concentration of 40% which caused the enzymes to denature.
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    PENGARUH PENAMBAHAN ION LOGAM Mg2+, Mn2+ DAN Ca2+ TERHADAP PERTUMBUHAN JAMUR Trichoderma asperellum LBKURCC1
    (perpustakaan UR, 2021-06) Prasetyorini, Hadisty Dian; Dahliaty, Andi
    Lacasse is an enzyme that requires additional chemical components (non protein) such as metal ions to increase its activity. This research aim to determine the effect of the addition of metal ions Mg2+, Mn2+ and Ca2+ to laccase enzyme activity produced by the Trichoderma asperellum LBKURCC1 using ABTS as a substrate. The concentration of each metal ions is 2 mM. The laccase enzyme activity assay was measured using a UV-Vis spectrophotometer with a wavelength of 420 nm and incubated for 0, 5 and 10 minutes. The highest laccase enzyme activity was obtained at 5 minutes incubation for the addition of Ca2+ metal ions, with the value is 3,7064 x 10-3 U/mL which is able to increase lacasse activity by 73,97%. The addition of Mn metal ions causes a decrease in laccase activity by 13.89% from the control with the resulting activity of 1.8611 x 10-3 and which is possible due to the effect of heavy metals.
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    PENGARUH pH DAN SUHU TERHADAP AKTIVITAS ENZIM LAKASE Trichoderma asperellum LBKURCC1 HASIL PENGENDAPAN 0-20% AMONIUM SULFAT
    (perpustakaan UR, 2021-06) Maswita, Risa; Dahliaty, Andi
    Laccase Trichoderma asperellum LBKURCC1 an oxidoreductase enzyme with the ability to oxidize various phenolic groups using oxygen as an electron acceptor. Laccase activity can be affected by pH and temperature. In this study, the determination of fractionated laccase activity was investigated in order to obtain higher laccase activity than crude extract activity. Determination of laccase activity was carried out using a spectrophotometer with a wavelength of 420 nm. The results the highest activity was obtained at pH 5,5 and a temperature of 60⁰C.
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    Penyuluhan Teknologi PembuatanSaus Tomat di Desa Sail Kecamatan Tenayan Raya Kotamadya Pekanbaru
    (2013-04-22) Linggawati, Amilia; Haryani, Yuli; Dahliaty, Andi; Fia Kartika, Ganis; Helza Yanti, Pepi
    Untuk menghasilkan saus tomat yang menarik minat pasar untuk membeli, produsen saus tomat menambahkan sejumlah bahan tambahan makanan. Penggunaan bahan tambahan makanan tersebut bertujuan untuk membuat makanan yang diproduksi tampak lebih berkualitas, tahan lama, menarik, serta memiliki rasa dan tekstur yang sempurna. Namun yang menjadi permasalahan adalah ketika terjadi pemakaian bahan tambahan makanan yang tidak sesuai dengan peraturan yang ada dan tidak adanya pengawasan dari pihak terkait. Penggunaan bahan tambahan makanan pada industri saus tomat telah banyak dilaporkan. Diantaranya adalah penelitian yang dilakukan oleh Siaka 2009, yang menunjukkan kandungan asam benzoat dalam saus tomat yang melebihi kadar maksimum yang diperbolehkan menurut Permenkes No. 722/Menkes/Per/IX/1988. Penelitian Mulyanti 2007 menunjukkan bahwa kandungan pewarna tartrazine telah melebihi standar yang diperkenankan SNI.
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    PRODUKSI SELULASE DARI BAKTERI ENDOFIT Pseudomonas stutzeri LBKURCC 54 DAN LBKURCC 59 MENGGUNAKAN SELULOSA POPOK BAYI SECARA FERMENTASI PADAT
    (2016-05-16) Amelia, Silfi; Devi, Silvera; Dahliaty, Andi
    Disposable diapers are made of cotton containing cellulose and can be used for the production of cellulase by solid state fermentation. Cellulase is an enzyme that can hydrolyze the β-1,4 glicosidic of cellulose and produce glucose. Cellulase can be produced by many microorganisms include endophytic bacteria Pseudomonas stutzeri LBKURCC 54 and LBKURCC 59 at fermentation time of 10, 20, 30, 40 and 50 days. The activity of cellulase was determined by the Nelson-Somogyi method. The results showed that highest cellulase activity of LBKURCC 54 was occurred at the 40th day of fermentation time (1.900 ± 0.346) x 10-3 U/mL, while the highest cellulose activity of LBKURCC 59 was occurred at the 30th day of fermentation time (3.530 ± 3.415) x 10-3U/mL.
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    Uji Antagonistik Dan Antijamur Media Produksi Kitinase Trichoderma asperellum TNJ63 Galur Lokal Riau Pada Beberapa Jamur Patogen Tanaman
    (2015-07-02) Dahliaty, Andi; Nurulita, Yuana; T. Nugroho, Titania
    Trichoderma asperellum TNJ63 adalah galur lokal Riau penghasil kitinase. Kitinase erat kaitannya dengan kemampuan melindungi tanaman terhadap beberapa penyakit tanaman karena kitinase adalah enzim yang mampu memecah kitin yang pada umumnya merupakan komponen diding sel jamur pathogen tanaman. Penelitian yang menguji aktivitas antagonistik dan antijamur dari media produksi kitinase terhadap beberapa jamur pathogen {Fusarium sp, Ganoderma boninens, dan Rhizoctonia solani) perlu dilakukan, sehingga dapat dijadikan studi pustaka pengembangan metode penanggulangan penyakit tanaman di Riau. Uji antagonistik dilakukan dengan dengan cara meletakan potongan jamur pathogen dan jamur uji di atas media agar yang berjarak 2cm, penghambatan diamati setelah 4, 5 dan 6 hari, sedangkan uji antijamur dengan metoda Fungal Growth Inhibition assay yaitu dengan cara meletakan potongan jamur pathogen diatas media yang mengandung enzim kitinase I x dan 2x kuat. Untuk mendapatkan enzim Ix dan 2x kuat enzim dipekatkan dengan menggunakan ultrafiltrasi.
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    UJI TOKSISITAS FRAKSI n-HEKSANA AREAL PART DARI TANAMAN Amaranthus spinosus L
    (2016-05-10) Puspitasari, Dwi; Dahliaty, Andi; Balatif, Nur
    Amaranthus spinosus L (Amaranthaceae) has long been used by Indonesian people and neighboring countries as a traditional medicine. In this study, toxicity of the aereal part of this plant has been conducted using Brine Shrimp Lethality Test (BSLT). The results of this test showed that LC50 values of hexane and methanol extract were 1,59 and 69.98 μg/mL respectively. Fraction of the hexane extract did not show any activities except fraction 2 with LC50 635,33 μg/mL.