UJI AKTIVITAS ANTIBAKTERI DAN TOKSISITAS EKSTRAK n-HEKSANA DAGING BIJI KLUWAK (Pangium edule Reinw)
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Date
2016-05-02
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Abstract
Kluwak (Pangium edule Reinw) is a plant found in Asia, especially in Indonesia. It has not
been studied comprehensively. The results of phytochemical test from seed flesh
(endosperm) of kluwak showed that this plant contains secondary metabolites alkaloids,
terpenoids, saponins, flavonoid, and phenolic. n-Hexane extract the endosperm separated
as oil seeds and analyzed using Gas Chromatography-Mass Spectroscopy (GC-MS).
The results of the analysis using gas chromatography showed 7 peaks and mass
spectroscopy analysis revealed a number of major constituents of oil compound in the form
of diethyl phthalate (2.49%), methyl palmitate (10.85%), cycloocta pyran-1,3,5,6,7,8,9,10-
hexahydro-4-isopropyl-1-phenyl (1,45%), methyl oleate (66.05%), and methyl stearate
(10.85%). The antibacterial activity were determined by agar diffusion method against
Bacillus subtilis (Gram-positive bacteria) and Escherichia coli (Gram-negative bacteria).
Biological data indicated that the extract has activity against both bacterial test with 7,1 mm
of diameter inhibition zone against the bacteria Bacillus subtilis and 6,9 -11,0 mm against
the bacteria Escherichia coli. Toxicity test was conducted using Brine Shrimp Lethality
Test (BSLT). The toxicity results showed that the n-hexane extract of the endosperm of
kluwak did not show potential as anticancer compounds proven with LC50 > 1000 ppm.
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Keywords
Bacterial test, Brine Shrimp Lethality Test (BSLT), GC-MS, kluwak (Pangium edule Reinw)