Abstract:
Fungi of the genus Trichoderma are soilbome, green-spored ascomycetes that
can found all over the world. Trichoderma sp. have been known to be able to attack
other fungi, to produce antibiotics that affect other microbes, and to acts as biocontrol
microbes. Trichoderma T.N.C52 and T.N.J63 are isolated from citrus and
cocoa plantation soil in Riau. Trichoderma LBKURCC 20 and LBKURCC 21 are
isolated from Hutan Giam Siak kecil, Bukit Batu. Species of Trichoderma producing
several fungitoxic cell-wall-degrading enzymes have also been adopted as agents of
biological control of plant pathogenic fungi. They produced the cellulases are
important to degradation cellulosic waste. In this research, enzyme produced by
fermentation experiments were performed in shake flasks using
Carboxymethylcellulose (CMC) as carbon sources and pH 5.5. The incubation time is
72 hours. The activity of enzyme test using CMC as substrate and various
temperature. At SS'^C, 40°C, and 45°C, activity of enzyme are (6.0542±5.623) x 10"^;
(7.3326±3.3444) x 10"^; dan (1.9958±1.4780) x 10"^ respectively.
