ISOLASI DAN UJI TOKSISITAS EKSTRAK METANOL DARI DAGING BIJI TANAMAN SIRSAK (Annona muricata Linn)

Abstract

Isolation and toxicity test of soursop (Annona muricata L) seed flesh have been done. The isolation of chemical compounds was done by maseration using n-hexane and methanol while its toxicity was determined by Brine Shrimp Lethality Test method using Artemia salina Leach. Gas Chromatography Mass Spectroscopy (GC-MS) was used to analyze the chemical compounds from methanol fraction. A total of 1100 g soursop seed flesh yielded 140.8 g of n-hexane extract and 108.6 g of methanol extract. The results of toxicity test showed that n-hexane extract has no toxicity (LC50 = 1122.02 ppm), while methanol extract has toxicity (LC50 = 0.59 ppm). The result of GC-MS analysis showed that the 6th fraction of methanol extract contained methyl palmitic (peak of 1.96 on 20.95 minutes retention time), methyl linoleic (peak of 2.12 on 22.99 minutes), and methyl oleic (peak of 2.23 on 23.05 minutes) based on NIST and WILEY 7 library.