Abstract:
Shoot induction is an early stage in plant propagation in vitro. The aim this of study was to determine the best BAP concentration and type of explants for shoot induction of
siam orange (Citrus nobilis Lour.) from Kampar using in vitro technique. The study had been conducted in the Laboratory of Integrated Biology, Department of Biology,
Faculty of Mathematics and Natural Science, University of Riau from May to June 2012, using randomized block design with factorial treatment. This experiment used two factors, the first factor was BAP concentration consisted of five levels: 0, 1, 2, 3, 4,
5 mg/l and the second factor was the type of explant (whole seed and cotyledon). The
explants were cultured in MS medium with 12 replications. The data was analyzed using Analysis Of Variance (ANOVA) and if the result was significant, this analysis was continued using Duncan Multiple Range Test (DMRT) at the level of 5%. The results of this study showed that the best shoot induction was obtained from whole seed
and cotyledon explant grown that on MS medium without BAP. The percentage and the
average number of shoot from whole seed explants were 100% and 4,08, respectively.
While the percentage and the average number of shoot from cotyledon explants were
91,67% and 1,50, respectively. Therefore the shoot induction from whole seed explant
better than cotyledon explant.
