Abstract:
Ethanol consumption of the world for a variety of uses has increased very significantly in recent years. Therefore it is necessary to alternate sources of raw materials to manufacture bioethanol and bioethanol production can be increased. Nipa sap is one of potential materials to be processed into bioethanol. Availability of nypa palm land in Indonesia and a fairly high sugar content (15-20%) makes nipa sap has the potential to be processed into bioethanol, so that produce an useful and high valuable product and then in education, that is give data process fermentation of nipa sap with comparison of pH and scale of fermentation. Through a fermentation process using yeast Saccharomyces cereviceae, glucose is converted into bioethanol and carbon dioxide. Starter preparation is done by the yeast Saccharomyces cereviceae inoculum in the 10% of fermentation medium so that yeast is able to adapt and ready for fermentation. Fermentation takes place in batches with a volume of 50 liters of fermentation medium, variations in the pH 4.5, 5.0, 5.5 and variations of fermentation time of 24, 36, 48, 60 dan 72 hours. The stirring speed of 200 rpm and temperature of fermentation at room temperature (25 - 30 0C). Ethanol concentrations were analyzed using alkoholmeter. The process of optimum fermentation conditions indicated in the addition of starter pH 4,5 and fermentation time of 36 hours of the initial sugar concentration of 221,163 mg/ml. Bioethanol concentration obtained in this condition is 14% (v/v) or 112,793 mg/ml with the acquisition of 97,969% yield.
