AKTIVITAS ANTIBAKTERI EKSTRAK DAUN PEPAYA (Carica papaya L.) TERHADAP Escherichia coli DAN Salmonella typhi
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Date
2013-03-04
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Abstract
Papaya (Carica papaya L.) is a plant which had been used by society since long time
ago as a traditional medicine. It was predicted that there was antibacterial substances in
papaya, so it can be used as antibiotic substitutes. The aims of this study were to know
the extraction method and concentration of Carica papaya L. against the test bacteria.
Carica papaya L. leaf extract were made by maceration method, grinding method,
boiling fresh leaf method, and boiling dry leaf method. The test bacteria were E. coli
and S. typhi. The inhibition zone was determined for concentration ranging from 10% to
100% (10%, 25%, 50%, 75%, 100%). Antibacterial activity test was done using paper
disc and agar well methods. The result showed that Carica papaya L. leaf extract had
antibacterial activities against E. coli and S. typhi that was showed by zone of inhibition.
Maceration method with agar well was the most effective extraction method against of
E. coli and S. typhi. The bigest zone of inhibition was showed in concentration 100%.
The maceration method with agar well demonstrated the highest activity against E. coli
(17±1 mm zone of inhibition) and S. typhi (15±0,5 mm zone of inhibition)
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Keywords
antibacteria, Carica papaya L., Escherichia coli, Salmonella typhi