Pengaruh 2.4 D Dan BAP terhadap Multiplikasi Tunas Eksplan Buah Naga (Hylocereuscostaricensis) Melalui Teknik Kultur Jaringan Secara Invitro

Abstract

PENGARUH 2.4 D DAN BAP TERHADAP MULTIPLIKASI TUNAS EKSPLAN BUAH NAGA (Hylocereus costaricensis) MELALUI TEKNIK KULTUR JARINGAN SECARA IN VITRO Riza Hanizah1, Imam Mahadi2, Sri Wulandari2 1Mahasiswa Program Studi Pendidikan Biologi 2Dosen Pendidikan Biologi FKIP Universitas Riau Fakultas Keguruan dan Ilmu Pendidikan Program Studi Pendidikan Biologi Universitas Riau-Pekanbaru (28293) email: hanizariza@gmail.com Abstrak This research aimed to determine the effect of 2.4 D and BAP on shoot multiplication explants dragon fruit (Hylocereus costaricensis). The experiments were performed using Complete Randomized Design (CRD) with factorial pattern. The first factor is 2.4 D which consists of three standard treatments of 0 ppm, ppm 0.25 and 0.5 ppm. The second factor is Benzyl Amino Purine (BAP), which also consists of 3 level of treatment that is 0 ppm, 3 ppm and 5 ppm. Parameters measured were time study appeared shoots, number of shoots regenerated (Shoots/ explants) and shoots high. The results were analyzed using one way ANOVAwith further test DMRT (Duncan's Multiple Range Test) at the level of 5%. The results showed treatment with 3 ppm BAP produced the fastest time of emerging shoots 14.5 HST and produces the highest average number of shoot /explant 5.38 buds. As for the best high of buds produced by combination of 0.25 ppm 2.4 D - 3 ppm BAP is the average height 1.74 cm. The best treatment for shoot multiplication explants dragon fruit is at a concentration of 0 ppm 2.4 D - 3 ppm BAP emerging shoots with average 14.05 HST (days after planting) the average number number of shoots regenerated (Shoots/ explants) 5.38 buds and the mean height 1.68 cm.