ISOLASI DAN UJI TOKSISITAS EKSTRAK n-HEKSANA DARI BENALU JENGKOL Scurrula ferrugimea Danser
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Date
2023-09
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Elfitra
Abstract
Scurrula ferruginea Danser is a semi-woody plant from the santalales family and
the Loranthaceae family. This plant is considered detrimental because it is a parasite
on other plants, but currently S. ferruginea has a lot of potential as a traditional
medicine. This research aims to isolate secondary metabolite compounds,
characterize the compounds, and analyze the toxicity test of the n-hexane extract of
the S. ferruginea plant using the brine shrimp lethality test (BSLT) method. The
extraction process was carried out using the maceration method with methanol
solvent followed by a partition process using n-hexane and ethyl acetate solvents to
obtain a thick n-hexane extract. Separation of components in the extract was carried
out using vacuum liquid chromatography (VLC) and column chromatography
methods. Fraction 2 from the results of column chromatography separation in the
form of crystals coded SFH-F2 has a melting point of 134-136°C. Characterization
using FTIR was obtained at wavenumber 3300 cm-1 (OH), 1451 cm-1 (CH2), 1380
cm-1 (CH3), 1189 cm-1 (CN), dan 1043 cm-1 and 1036 cm-1 (CO). Characterization
using UV-Vis did not show any absorption. Based on the characterization results,
the compound SFH-F2 is a terpenoid group. The results of toxicity testing of nhexane
extract using the BSLT method obtained an LC50 value > 1000 ppm. These
results indicate that the n-hexane extract of the S. ferruginea plant is non-toxic.
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isolation, characterization, toxicity
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