Abstract:
Penicillium sp. LBKURCC34 and LBKURCC38 are fungi isolated from peat soil
of the primary forest of the Giam Siak Kecil Bukit Batu Biosphere Reserve
(GSKBB) of Riau Province. This fungus is known to have the potential to produce
secondary metabolites that have antimicrobial activity. This research was
conducted using co-culture technique between Penicillium sp. LBKURCC34 with
Penicillium sp. LBKURCC38. Extraction was carried out using ethyl acetate
(EtOAc), butanol (BuOH) and acetone (Ase) solvents. These extracts were tested
for their antimicrobial activity using the disc diffusion method. The results of the
antimicrobial activity test showed that the EtOAc extract at a concentration of 2000
μg/disc had antimicrobial activity against four pathogenic microbes namely S.
epidermidis, B. subtilis, E. coli, and C. albicans, BuOH extract only had
antimicrobial activity against 2 pathogenic microbes namely B. subtilis and C.
albicans, whereas at a concentration of 200 μg/disc, the EtOAc extract only had
antimicrobial activity against pathogenic microbes S. aureus and C. albicans.
BuOH extract only has activity against S. aureus bacteria, while Ase extract only
has activity against C. albicans fungus.
