Abstract:
Fungi strain LBKURCC304 is a thermophilic fungi isolated from Bukik Gadang hot
spring, West Sumatera, can produce amylase enzyme based on semi-quantitatively and
quantitatively analysis. Morphological characteristics indicated that strain
LBKURCC304 was a member of Aspergillus genera. Morphological identification
cannot be used to determine species of fungi, therefore molecular identification is
required. Determination of species is very important to explore the potential of
LBKURCC304 with reference from the same species. Molecular identification of
Aspergillus sp. LBKURCC304 was initiated by isolation of genomic DNA from 2 days
old mycelia, PCR amplification on ITS-1 and ITS-2 rDNA regions were successfully
performed using ITS4 and ITS5 primers at annealing temperatures of 45ºC. The
sequences were verified with BioEdit program and then aligned to get the complete
sequences. DNA sequences of Aspergillus sp. LBKURCC304 were analyzed using
BLAST. The genomic DNA and PCR product have molecular weights of 16.425 bp and
593 bp, respectively. The BLAST analysis showed that Aspergillus sp. LBKURCC304
is Aspergillus fumigatus.
