Abstract:
Isolation of active coumpound of root Spatoglottis aurea Lindl was done with maceration method using methanol. The methanol extract was partitioned with hexane and obtained a total of methanol extract and hexane. Extract methanol and hexane were fractionated with column chromatography and were obtained separation results 4 fractions that is Fm1-Fm4 and Fh1-Fh4 and the fractions 2 (Fh2) of hexane extract was obtained white crystals. From UV spectra of crystal Fh2 showed there was a transition π →π* indicating that there was Fh2 crystal C=C double bond. Fh2 IR spectrum of the compound showed OH group, GC-MS analysis showed the compound has the formula of C29H50O with a peak molecular ion m/z 414 which was a coumpound of β-sitosterol with a melting poin of 131-133 0C. Antibacterial activity test extract of methanol and hexane were not active against bacteria test, while the fraction of Fh1 and Fh2 hexane extract active against bacteria S. aureus dan bakteri E. coli.
