Abstract:
Fluorescence imaging is a method used to detect the early effects of environmental conditions on
plants before the greater damage on plants happen. A fluorescence imaging optical system was
built in this research consisted of two LEDs with different wavelength i.e. 450 nm and 680 nm, 3
MP CMOS camera and lens, and ND filters (neutral density filter). The intensities of LED light
were varied using three different ND filters which were represented by their optical power. The
samples were Amaranthus tricolor L. Spinach varitas. The plants were five different water volume
i.e. 75 ml, 100 ml, 125 ml, 150 ml,and 175 ml. The differences between chlorophyll fluorescence
intensity of the spinach leaves for all treatments were investigated. The images of leaves after
illuminated by the LED lights were recorded by the CMOS camera. The fluorescenced intensities
were found from RGB plot using ImageJ software. The research results show that the maximum
fluorescence intensity spinach which grow with water volume 75 ml and the minimum
fluorescence intensity spinach which grow with water volume 125 ml used LED with wavelength
450 nm and 680 nm. The difference of fluorescence intensity spinach which grow with water
volume 75 ml and 125 ml used LED at wavelength 680 nm was seen higher when LED intensity
was the lowest which was about 45,32 % difference.
