EFEK EKSTRAK ETANOL DAUN PELAWAN (Tristaniopsis obovata R.Br) TERHADAP STRUKTUR GINJAL TIKUS PUTIH (Rattus norvegicus) YANG MENGALAMI UROLITHIASIS

Abstract

Urolithiasis is a kidney stones disease caused by urine-substances accumulation in stone formation. This research aimed to review the influence and the effective dose of pelawan leaf (Tristaniopsis obovata R.Br) ethanol-extract towards kidney hystological change of Rattus norvegicus. Experiments was designed using the post test only control group. Twelve of R. norvegicus was divided into 4 groups of treatment with 3 replications. Group I were treated with inducer (etilen glikol 0.75% dan amonium klorida 2%) to form kidney stone. Group II and group III were treated with inducer and pelawan leaf ethanol-extract with dose of 100 mg/kg BW and 150 mg/kg BW for each group, respectively. Control group were not treated with pelawan leaf ethanol-extract. All treatment was given orally for 14 days. Right and left kidneys of R. norvegicus were collected and processed to histological slide using paraffin method and were stained with Hematoxylin Eosin (HE). The result showed that treatment of ethylene glycol caused cell swelling in glomerulus (100%), proximal tubule (67,7%), distal tubule (71.3%), and the loop of Henle (75,8%). Additionally, ethylene glycol also caused cell necrosis (36.1%). The treatment of pelawan leaf ethanol-extract with dose of 150 mg/kg BB is more capable to protect cell swelling in glomerulus (35%), proximal tubule (35,5%), distal tubule (18,4%), the loop of Henle (34,9%) and cell necrosis (15,4%).