Abstract:
The genus Botryosphaeria, which can flourish in a range of climatic conditions
worldwide, includes the endophytic fungus Botryosphaeria rhodina. This genus's
antimicrobial activity has been reported in several of its species. This investigation aims
to assess the antibacterial activity of an endophytic fungus, B. rhodina, from the
mangrove root, Xylocarpus granatum J. Koenig, against Gram-positive and Gramnegative
bacteria, including Listeria monocytogenes ATCC 7644, Salmonella
typhimurium ATCC 14028, and Bacillus subtillis ATCC 19659. With a clear zone
diameter ranging from 16 to 22 mm, the results of the antibacterial test using the well
diffusion method revealed that the methanol extract at a concentration of 500 μg/mL had
antibacterial activity in the strong to very strong category for all pathogenic bacteria
tested. The L. monocytogenes bacteria had the largest clear zone, measuring 22.03 mm
by 0.30 mm.
