Abstract:
Bacterial isolates named LBKURCC419, LBKURCC423 and LBKURCC424 are natural forest
soil bacteria isolated using tetracycline and amoxicillin media from the Giam Siak Kecil Bukit
Batu Biosphere Reserve, Riau Province. In this research, these bacteria were assessed by
molecular biological techniques to know whether the isolates contain antibiotic resistance genes
especially tet (tetracycline). The bacterial chromosomal DNA was isolated using the TIANamp
DNA bacteria kit, then continued to determine the genes encoding tetracycline resistance using
polymerase chain reaction (PCR) with eight primers of the tet genes according to the respective
annealing temperature. PCR products were identified by electrophoresis. The results showed the
eight tet genes were identified negative that meant the isolates did not have that gene. This can
occur because the tested resistant genes may be present in plasmids while in this study perhaps
the isolated DNA came from chromosomes so that it did not contain the resistant genes tested. In
addition, the nature of low resistance to bacteria because it comes from natural soil also causes
the mechanism of bacterial resistance to be unknown.
