Abstract:
Durik-durik (Syzygium sp.) is a plant from the family Myrtaceae that can grow in
the flooded swamp ecosystem. This plant has a good adaptability to inundation
stress. This study aimed to determine the optimal annealing temperature that could
be used for amplification in the atpB-rbcL intergenic spacer region. Leaf samples
of durik-durik (Syzygium sp.) plant were taken from Lake Kajuik located in
Langgam Village, Pelalawan Regency, Riau Province. Samples were extracted
using Genomic DNA mini kit Plant (Geneaid) then amplified using dreamTaq
DNA polymerase and atpB-rbcL intergenic spacer primer pair with a PCR
machine. The results showed that DNA bands were around 1000 bp. The optimal
annealing temperature was Tm-5 = 46,6 oC.
