ISOLASI DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETIL ASETAT DAUN Nerium oleander

Abstract

Isolation and an antioxidant activity assay from extracts ethyl acetate of Nerium oleander leaves had been done. The extracts was obtained by macerating dried leaves powder of N. oleander with n-hexane and ethyl acetate. The ethyl acetate extract was fractionated by vacuum liquid chromatography (VLC) and the combined fractions 6 (Fg6) of VLC was continued to be fractionated by using column chromatography. An antioxidant activity quantitatively (ethyl acetate extracts and fractions of vial number 35 of column chromatography) and qualitatively (Fg6 of VLC) had been done by using DPPH (1,1-diphenyl–2–picryl hydrazyl) method. Fraction of the vial number 35 of column chromatography chosen was analyzed and identified by Ultraviolet Visible spectroscopy (UV-Vis), Fourier Transform Infrared (FT-IR) and HPLC (High Performance Liquid Chromatography). Analysis of the fractions vial number 35 in IR spectrum showed OH, aliphatic CH, C = O groups, and HPLC chromatograms showed that the compound was not pure. The results of antioxidant activity showed the extract of ethyl acetate (IC50 = 360.98 g/mL) was active as an antioxidant compared to fraction of the vial number 35 of column chromatography (IC50 ≥ 1000 g/mL), and Fg 6 of VLC was active as an antioxidant.