ISOLASI DAN KUANTIFIKASI RNA TOTAL PADA SAMPEL LIVER YANG DIINDUKSI D-GALN/LPS DENGAN METODE SPEKTROFOTOMETRI
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Date
2023-06
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Elfitra
Abstract
Liver disease is a serious global health issue. One of the causes of liver disease is
hepatitis, which is the seventh leading cause of death worldwide. Research on drugs
for liver damage cases has been extensively conducted. The effectiveness and
toxicity of a compound on liver cells can be tested by analyzing gene expression,
which starts with the isolation and quantification of RNA. The isolation process is
carried out to obtain RNA, which carries genetic information, while quantification
is performed to determine the concentration and purity of the isolated RNA. In the
research conducted, the isolation was performed using the trizol method, and
quantification was done using the spectrophotometry method. Based on the research
findings, a significant amount of RNA concentration and good RNA purity were
obtained, enabling its use for further analysis.
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Keywords
isolation, quantification, RNA
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